Time ago I wrote about my problem, but I've been sick and don't resolve it.
From Frame I make wxSplitterWindow, and after that from menu I want to put in each SplitterWindow - wxTreeCtrl.
I get from examples class pyTree(wx.wxTreeCtrl) , which works fine.
Now in each splittered frame in left top corner I see minimaized point of tree.
I think it happend becouse I don't put correctly SetSize or SetSizer, I tried to do it but without any success.
My opinion confirmed Robin Dunn, he wrote:
You also need to give self.p1 and self.p2 sizers, and add the tree's to those sizers when they are created, and finally, call self.p1.Layout and self.p2.Layout so the sizers will be triggered and will size/position the tree's.
If any have a bit of text with this solution I will be grateful. 
Leonid